Copper isotope compositions of superoxide dismutase and metallothionein from post-mortem human frontal cortex

F Larner; CA McLean; AN Halliday; BR Roberts

Journal article

Copper isotope compositions of superoxide dismutase and metallothionein from post-mortem human frontal cortex

F Larner, CA McLean, AN Halliday, BR Roberts

Inorganics | MDPI | Published : 2019

DOI: 10.3390/INORGANICS7070086

Abstract

The natural copper isotopic compositions of superoxide dismutase and metallothionein from six post-mortem human frontal cortices were determined using a combination of size exclusion protein liquid chromatography, followed by anion exchange chromatography and multiple collector inductively-coupled plasma mass spectrometry. Superoxide dismutase was enriched in the heavier65Cu relative to the metallothionein fraction in all specimen pairs. The isotopic compositions were independent of copper content. This finding provides evidence that nitrogen ligands in protein copper binding sites will be enriched in heavy metal isotopes, and sulphur ligands will preferentially incorporate lighter isotopes ..

View full abstract

University of Melbourne Researchers

Catriona McLean Author

Related Projects (2)

HARNESSING THE CONSEQUENCES OF IMPAIRED MITOCHONDRIAL ENERGY METABOLISM TO TREAT THE SYMPTOMS OF AGE-RELATED NEURONAL DECLINE

Cognitive function and locomotor ability decline due to advanced age and disorders such as motor neuron disease, Alzheimer’s and Parkinson’s..

Metalloproteomics: A new piece of the systems biology puzzle

Systems biology uses advanced analytical technology to study the complex chemistry of the living cell. Many cellular functions are the resul..

Grants

Awarded by Seventh Framework Programme

Funding Acknowledgements

This study was funded through Royal Society International Exchanges Grant IE140089 to F.L. and a Science and Technology Facilities Council (STFC) Grant (ST/M001318/1) and European Research Council (ERC) Advanced Fellowship (247422) to A.N.H. We acknowledge funding from the Victorian Government's Operational Infrastructure Support Program and the Australian Research Council Linkage Projects Scheme to B.R.R. LP140100095 (with Agilent Technologies). Partial support is acknowledged from the National Health and Medical Research Council (1061550 and 1138673), the Cooperative Research Centre for Mental Health, the Motor Neuron Disease Research Institute of Australia, and the Neuroproteomics Facility.